Anti-ANXA1, Rabbit, Polyclonal

Catalog Number: ATA-HPA011272
Article Name: Anti-ANXA1, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA011272
Supplier Catalog Number: HPA011272
Alternative Catalog Number: ATA-HPA011272-25,ATA-HPA011272-100
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: ANX1, LPC1, Pan-Cancer
annexin A1
Anti-ANXA1
Clonality: Polyclonal
Concentration: 0.1 mg/ml
Isotype: IgG
NCBI: 301
UniProt: P04083
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNQRQQIKAAYLQETGKPLDETLKKALTG
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: ANXA1
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:500 - 1:1000, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry analysis in human esophagus and cerebral cortex tissues using HPA011272 antibody. Corresponding ANXA1 RNA-seq data are presented for the same tissues.
Immunohistochemical staining of human cerebral cortex, colon, esophagus and liver using Anti-ANXA1 antibody HPA011272 (A) shows similar protein distribution across tissues to independent antibody HPA011271 (B).
Immunohistochemical staining of human lung shows strong positivity in macrophages.
Immunohistochemical staining of human lymph node shows moderate positivity in non-germinal center cells.
Immunohistochemical staining of human colon shows strong positivity in lymphoid cells.
Immunohistochemical staining of human esophagus shows strong positivity in squamous epithelial cells.
Immunohistochemical staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemical staining of human liver using Anti-ANXA1 antibody HPA011272.
Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ANXA1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western blot analysis using Anti-ANXA1 antibody HPA011272 (A) shows similar pattern to independent antibody HPA011271 (B).
Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
HPA011272
HPA011272
HPA011272