Anti-SMAD4, Rabbit, Polyclonal

Product Overview

• Article Name: Anti-SMAD4, Rabbit, Polyclonal
• Biozol Catalog Number: ATA-HPA019154
• Supplier Catalog Number: HPA019154
• Alternative Catalog Number: ATA-HPA019154-25,ATA-HPA019154-100
• Manufacturer: Atlas Antibodies
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
• Conjugation: Unconjugated
• Alternative Names: DPC4, MADH4, Pan-Cancer

Product Description

SMAD family member 4

Additional Information

Anti-SMAD4

Product Properties

• Clonality: Polyclonal
• Concentration: 0.3 mg/ml
• Isotype: IgG
• NCBI: 4089
• UniProt: Q13485
• Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
• Purity: Affinity purified using the PrEST antigen as affinity ligand
• Sequence: AFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESTSTANFPNIPVASTSQPASILGGSHSEGLLQI
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
• Target: SMAD4
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:50 - 1:200, WB: 0.04-0.4 µg/ml

Images

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & centrosome.

Immunohistochemical staining of human rectum shows strong positivity in glandular cells.

Western blot analysis in human cell line SCLC-21H.

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

HPA019154

HPA019154

HPA019154