Anti-KRT8, Rabbit, Polyclonal
Catalog Number:
ATA-HPA049866
- Images (9)
| Article Name: | Anti-KRT8, Rabbit, Polyclonal |
| Biozol Catalog Number: | ATA-HPA049866 |
| Supplier Catalog Number: | HPA049866 |
| Alternative Catalog Number: | ATA-HPA049866-100 |
| Manufacturer: | Atlas Antibodies |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IHC, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence |
| Conjugation: | Unconjugated |
| Alternative Names: | CARD2, CK8, CYK8, K2C8, K8, KO, Pan-Cancer |
| keratin 8 |
| Anti-KRT8 |
| Clonality: | Polyclonal |
| Concentration: | 0.05 mg/ml |
| Isotype: | IgG |
| NCBI: | 3856 |
| UniProt: | P05787 |
| Buffer: | 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. |
| Purity: | Affinity purified using the PrEST antigen as affinity ligand |
| Sequence: | SFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK |
| Storage: | Store at +4°C for short term storage. Long time storage is recommended at -20°C. |
| Target: | KRT8 |
| Antibody Type: | Monoclonal Antibody |
| Application Dilute: | ICC-IF: 0.25-2 µg/ml, IHC: 1:1000 - 1:2500, WB: 0.04-0.4 µg/ml |
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Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments. |
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Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells. |
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Western blot analysis in human cell lines MCF-7 and SK-MEL-30 using Anti-KRT8 antibody. Corresponding KRT8 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1. |
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Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted) Lane 5: Human liver tissue Lane 6: Human tonsil tissue |
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