Anti-HSPA1A, Rabbit, Polyclonal

Catalog Number: ATA-HPA052504
Article Name: Anti-HSPA1A, Rabbit, Polyclonal
Biozol Catalog Number: ATA-HPA052504
Supplier Catalog Number: HPA052504
Alternative Catalog Number: ATA-HPA052504-25,ATA-HPA052504-100
Manufacturer: Atlas Antibodies
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Conjugation: Unconjugated
Alternative Names: HSP70-1, HSPA1, Pan-Cancer
heat shock 70kDa protein 1A
Anti-HSPA1A
Clonality: Polyclonal
Concentration: 0.2 mg/ml
Isotype: IgG
NCBI: 3303
UniProt: P0DMV8
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Purity: Affinity purified using the PrEST antigen as affinity ligand
Sequence: ACERAKRTLSSSTQASLEIDSL
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Target: HSPA1A
Antibody Type: Monoclonal Antibody
Application Dilute: ICC-IF: 0.25-2 µg/ml, IHC: 1:500 - 1:1000, WB: 0.04-0.4 µg/ml
Immunofluorescent staining of human cell line CACO-2 shows localization to vesicles.
Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferous ducts.
Immunohistochemical staining of human prostate shows moderate nuclear positivity in a subset of glandular cells.
Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251 MG
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
HPA052504
HPA052504
HPA052504