MolBoolean | BIOZOL

Article Name:	MolBoolean
Biozol Catalog Number:	ATA-MOLB00001
Supplier Catalog Number:	MOLB00001
Alternative Catalog Number:	ATA-MOLB00001
Manufacturer:	Atlas Antibodies
Category:	Kits/Assays

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MolBoolean™ Mouse/Rabbit is a kit for in situ protein proximity analysis in tissue and cells. The MolBoolean™ assay utilizes a proprietary oligonucleotide setup that enables the simultaneous detection of both free and interacting (~40 nm proximity) fractions for two proteins of interest (protein A, protein B and interaction proteins AB).

MolBoolean™ Mouse/Rabbit should be performed with the user’s primary antibodies of choice (raised in mouse and rabbit). MolBoolean™ relies on anti-mouse and anti-rabbit secondary proximity probes and rolling circle amplification (RCA) as a mean to amplify signal.

A series of molecular steps, performed in the MolBoolean™ assay, incorporates information into the amplified products indicating antibody target engagement of individual versus interacting proteins. This information is converted to fluorescent signals by the binding of detection reporters, allowing visualization on a conventional fluorescent microscope. An image analysis software is provided to segment and differentiate fluorescent signals, enabling the relative quantification of free versus interacting fractions for the two analyzed protein targets in the sample.

Unlock the secrets of protein-protein interactions in cells and tissue with MolBoolean™

MolBoolean™ is a novel in situ proximity technology developed by Atlas Antibodies that enables the simultaneous detection of both free and interacting fractions for two protein targets.

Complete spatial quantitative analysis of protein-protein interactions by simultaneous detection of free and interacting proteins.
Accurate quantification by normalization of interaction data to total target protein levels.
Biologically relevant data without the need for engineered protein expression.
1000-fold increased fluorescence signal by Rolling Circle Amplification, allowing detection and quantification of low abundant proteins.
Adaptable to different research needs. Universal kit that can be used with the customer’s choice of primary antibodies.
Validated in both cells and tissue.

Included in the Kit: Antibody proximity probes, oligonucleotides, enzymes, fluorescent detection reporters.
Not included in the Kit: Primary antibodies.
Target Species: Mouse, Rabbit.
Kit Size: 4.8 ml, approximately 120 assays (40 µl/assay).

Storage:

- 20 °C

	MolBoolean Kit (ATA-MOLB00001)
	E-cadherin/ β-catenin MolBoolean staining in MCF7 cells MolBoolean analysis of the interaction between E-cadherin (AMAb90862, magenta) and β-catenin (HPA029159, green) in MCF7 cells, showing the relative quantification of free versus interacting protein fractions, indicated by the detection of rolling circle products (RCPs) in either one or two fluorescent channels. Data is normalized to total target protein levels (total RCPs).
	EMD/LMNB1 MolBoolean staining in MCF7 cells MolBoolean analysis of the interaction between EMD (AMAb90562, magenta) and LMNB1 (HPA050524, green) in MCF7 cells, showing the relative quantification of free versus interacting protein fractions, indicated by the detection of rolling circle products (RCPs) in either one or two fluorescent channels. Data is normalized to total target protein levels (total RCPs).
	ACE2/TMPRSS2 MolBoolean staining in human kidney MolBoolean analysis of the interaction between ACE2 (AMAb91259, magenta) and TMPRSS2 (HPA035787, green) in human kidney, showing the relative quantification of free versus interacting protein fractions, indicated by the detection of rolling circle products (RCPs) in either one or two fluorescent channels. Data is normalized to total target protein levels (total RCPs).
	SATB2/HDAC1 MolBoolean staining in human colon MolBoolean analysis of the interaction between SATB2 (AMAb90682, magenta) and HDAC1 (HPA029693, green) in human colon, showing the relative quantification of free versus interacting protein fractions, indicated by the detection of rolling circle products (RCPs) in either one or two fluorescent channels. Data is normalized to total target protein levels (total RCPs).