oYo-Link SureLight PE Antibody Labeling Reagent - Labels 1mg

Product Overview

• Article Name: oYo-Link SureLight PE Antibody Labeling Reagent - Labels 1mg
• Biozol Catalog Number: ATR-ATSL001-1000
• Supplier Catalog Number: ATSL001-1000
• Alternative Catalog Number: ATR-ATSL001-1000
• Manufacturer: AlphaThera
• Category: Antikörper

Product Description

PE is an ultra-bright fluorophore isolated from a group of red photosynthetic pigments found primarily in red algae that emits an intensely bright red-orange fluorescence. PE conjugates are most commonly used for applications requiring high sensitivity s

Additional Information

oYo-Link® SureLight® PE enables site-specific labeling of compatible antibodies with up to two labels of PE for FACS, flow cytometry, and multimer/tetramer applications.

Simply mix the oYo-Link® SureLight® PE with the desired antibody followed by photocrosslinking for conjugation in 30-sec hands on time, 2 hours total. 

Requirements: Photo-Crosslinking Device

For research use only.

 

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PE is an ultra-bright fluorophore isolated from a group of red photosynthetic pigments found primarily in red algae that emits an intensely bright red-orange fluorescence. PE conjugates are most commonly used for applications requiring high sensitivity such as flow cytometry, ELISAs and microarray assays. 

This product is manufactured using SureLight® RPE which is a product of Columbia Biosciences, and uses a proprietary non-destructive cross-linking process that increases the thermal stability while maintaining fluorescent and spectral integrity of the native dye.

Generate antibody-PE conjugates in only 30-seconds hands on time, 2 hours total.

The antibody labeling procedure simply requires mixing oYo-Link® SureLight® PE with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm). 

Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table).

Applications: For fluorescence-based detection. Applications that do not require photostability.

Excitation/Emission: 565nm/574nm

Requirements: To label your compatible antibodies with oYo-Link®, you will need a light source emitting at 365nm (6-10W). Purchase AlphaThera’s Photo-Crosslinking Device, or use a compatible device.

 

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Specifications:

• Product Name:

  oYo-Link® SureLight® PE

• Catalog #:

  ATSL001

• Lot #:

  See the product spec sheet shipped with the product.

• Antibody and Buffer Compatibility:

  View the oYo-Link Antibody & Buffer Compatibility Table here.

  Note: To label mouse IgG1 antibody, you must order the oYo-Link mIgG1 products, which will only label mIgG1 antibody.

• Molar Concentration:

  33 µM

• Storage Buffer:

  1 x PBS

• Shipping Condition:

  This product is shipped as red liquid with cold gel packs.

• Reconstitution:

  No reconstitution required. Before opening, briefly centrifuge each tube to ensure that the entire solution is at the bottom.

• Storage and Stability:

  The product should be stored at 2-8°C in the dark. Do not freeze.

• Dye Lot #:

  RPE3000-24-039

  (from Columbia Biosciences)

• Fluorescence:

  Peak excitation (approximate): 565 nm

  Peak emission (approximate): 574 nm

• Other Notes:

  Disposal of product should be performed in compliance with all applicable federal, state, and local regulations. This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.

Product Properties

• Tag: PE
• Buffer: PBS
• Form: Liquid

Images

oYo-Link® SureLight® PE Flow Cytometry Data