oYo-Link SureLight APC Antibody Labeling Reagent - Labels 500ug

Product Overview

• Article Name: oYo-Link SureLight APC Antibody Labeling Reagent - Labels 500ug
• Biozol Catalog Number: ATR-ATSL002-500
• Supplier Catalog Number: ATSL002-500
• Alternative Catalog Number: ATR-ATSL002-500
• Manufacturer: AlphaThera
• Category: Antikörper
• Application: FACS, FC

Product Description

APC is an ultra-sensitive phycobiliprotein that exhibits far-red fluorescence (absorbs at 653nm and emits at 660nm). The intense brightness of APC means it is popular for flow cytometry/FACS and T-cell labeling. Excitation/Emission: 653nm/660nm SureLight

Additional Information

oYo-Link® SureLight® APC enables site-specific labeling of compatible antibodies with up to two labels of APC (allophycocyanin) for flow cytometry/FACS and T-cell labeling.

Simply mix the oYo-Link SureLight APC with the desired antibody followed by photocrosslinking for conjugation in 30-sec hands on time, 2 hours total. 

Requirements: Photo-Crosslinking Device

For research use only.

 

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APC is an ultra-sensitive phycobiliprotein that exhibits far-red fluorescence (absorbs at 653nm and emits at 660nm). The intense brightness of APC means it is popular for flow cytometry/FACS and T-cell labeling.

This product is manufactured using SureLight® Allophycocyanin which is a product of Columbia Biosciences, and uses a proprietary non-destructive cross-linking process that increases the thermal stability while maintaining fluorescent and spectral integrity of the native dye.

Generate antibody-APC conjugates in only 30-seconds hands on time, 2 hours total.

The antibody labeling procedure simply requires mixing oYo-Link® SureLight® APC with the desired antibody followed by photocrosslinking with non-damaging black light (365 nm) for 2 hours. 

Photocrosslinking can be carried out in most buffers, including those containing amine-containing molecules (e.g. TRIS, glycine) or storage proteins (see Buffer Compatibility Table).

Excitation/Emission: 653nm/660nm

Applications: Flow Cytometry/FACS, T-cell labeling.

Requirements: To label your compatible antibodies with oYo-Link®, you will need a light source emitting at 365nm (6-10W). Purchase AlphaThera’s Photo-Crosslinking Device, or use a compatible device.

 

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Specifications:

• Product Name:

  oYo-Link® APC

• Catalog #:

  ATSL002

• Lot #:

  See the product spec sheet shipped with product.

• Antibody and Buffer Compatibility:

  View the oYo-Link Antibody & Buffer Compatibility Table here.

  Note: To label mouse IgG1 antibody, you must order the oYo-Link mIgG1 products, which will only label mIgG1 antibody.

• Molar Concentration:

  33 µM

• Storage Buffer:

  1 x PBS

• Shipping Condition:

  This product is shipped as blue liquid with cold gel packs.

• Reconstitution:

  No reconstitution required. Before opening, briefly centrifuge each tube to

  ensure that the entire solution is at the bottom.

• Storage and Stability:

  The product should be stored at 2-8°C in the dark. Do not freeze.

• Conjugation Instructions:

  After reconstitution, each product tube is capable of labeling up to 100 µg of antibody. Please find and follow the Antibody Conjugation User Manual here.

• Dye Lot #:

  APC000-24-125

  (from Columbia Biosciences)

• Fluorescence:

  Peak excitation (approximate): 653 nm

  Peak emission (approximate): 660 nm

• Other Notes:

  Disposal of product should be performed in compliance with all applicable federal, state, and local regulations. This product does not meet hazard classification criteria based on evaluations made by our company under the OSHA Hazard Communication Standard, 29 CFR 1910.1200.

Product Properties

• Tag: APC
• Buffer: PBS
• Form: Liquid

Images

oYo-Link® SureLight® APC Flow Cytometry Data