Anti-Green Fluorescent Protein Antibody
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| Article Name: | Anti-Green Fluorescent Protein Antibody |
| Biozol Catalog Number: | AVL-GFP-1010 |
| Supplier Catalog Number: | GFP-1010 |
| Alternative Catalog Number: | AVL-GFP-1010 |
| Manufacturer: | Aves Labs |
| Category: | Antikörper |
| Application: | ICC, IHC, WB |
| Anti-Green Fluorescent Protein (GFP) Chicken Polyclonal Primary Antibody |
The Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house and detects Green Fluorescent Protein (GFP) and related variants including eGFP, YFP, CFP and others. It is a mixture of IgY fraction and affinity-purified antibodies and is great for use in ELISA, IHC, ICC, and WB.
BackgroundGreen Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.
Product DetailsAntigen: Recombinant GFP expressed in Escherichia coli Clonality: Polyclonal Host Species: Chicken Format: IgY Fraction Concentration: 10 mg/mL Physical State: Liquid Molecular Weight: 27 kDa Antibody Registry ID: AB_2307313
Applications: ELISA, ICC, IHC, WB Dilution Ranges WB: 1:5000-1:10000
Production Notes Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL). Quality Control Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans. |
| Clonality: | Polyclonal |
| Concentration: | 10 mg/mL |
| Buffer: | Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent. |
| Storage: | Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. |
| Antibody Type: | Primary Antibody |
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Staining of a tissue section through the midbrain region of an adult mouse for GFP (green) and Tryptophan Hydroxylase-positive neurons (red). |
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Pyramidal neurons in the hippocampal formation of a neonatal mouse brain. Tissue was paraformaldehyde-fixed (4%) and paraffin-embedded. GFP staining is in green in left panel. |
