Full length SARS-CoV-2 Spike Protein is single span transmembrane protein which contains the Spike protein amino acids 1-1273. Full length SARS-CoV-2 Spike Protein is manufactured in HEK293 mammalian cells, ensuring the most authentic post-translational modifications.
Single span transmembrane membrane protein (aa 1 – 1273)
Furin cleavage site RRAR mutated to GSAS
Trimerization (3 x 142 kDa)
Expressed in HEK293 Expi cells
C-terminal Rho1D4 tag for affinity purification
Solubilization and stabilization in LMNG detergent
2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent
Species: 2019-nCoV, COVID-19. Sequence: Full-length sequence (aa 1 – 1273), furin cleavage site “RRAR” mutated to “GSAG”; C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence. Accession No.: YP_009724390.1 (GenBank), P0DTC2 (UniProt). Tag: C-terminal Rho1D4 tag for affinity purification. Host: Expressed in HEK293 Expi cells. Applications: ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and Biophysical analysis. Purity: > 98% by SDS-PAGE gel. Trimerisation: 3 x 142 kDa. Includes transmembrane domain, a detergent screen was performed. Purification: 2-step purification via Rho1D4 tag and size exclusion chromatography in LMNG detergent. Confirmed by SDS-PAGE, native gel, Western Blot. Formulation: Liquid – Purified protein (1 mg/ml) supplied in 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG.
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