Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
Boster Bio Anti-Phospho-Potassium Chloride Cotransporter 2 (KCC2) (Ser940) SLC12A5 Antibody (Catalog P04629). Tested in WB, IP applications. This antibody reacts with Mouse, Rat.
Clonality:
Polyclonal
Concentration:
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Purity:
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
Form:
Liquid
Application Dilute:
WB: 1:1000IP: 40ul of antibody per 1mg lysate
Application Notes:
Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
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