The Nrf2 Luciferase Reporter cell line is a stably transfected MCF7 cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the antioxidant response element (ARE). ARE is known to regulate expression and induction
Buffer:
Each vial contains 2 ~ 3 x 10 6 cells in 1 ml of 90% FBS + 10% DMSO.
Application Dilute:
<h2>Application:</h2> Monitor the Nrf2 induction activity.Screen for activators or inhibitors of the Nrf2 signaling pathway.<h2>Culture conditions:</h2>Cells should be grown at 37C with 5% CO2 using Eagles Minimum Essential Medium
Application Notes:
Functional Assay, detecting the transcriptional activity of Nrf2
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