CytoLiner(TM) 650/675 Fixed Cell Membrane Stain, 250 labelings
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| Article Name: | CytoLiner(TM) 650/675 Fixed Cell Membrane Stain, 250 labelings |
| Biozol Catalog Number: | BOT-30134-T |
| Supplier Catalog Number: | 30134-T |
| Alternative Catalog Number: | BOT-30134-T |
| Manufacturer: | Biotium |
| Category: | Zellen/Zellkultur |
| CytoLiner(TM) Fixed Cell Membrane Dyes are novel lipophilic fluorescent dyes developed specifically for use in fixed and mildly permeabilized cells, compatible with later antibody staining. CytoLiner(TM) 650/665 has far-red fluorescence. Kit includes 50 uL of |
CytoLiner™ Fixed Cell Membrane Dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.
Features
Kit components
Novel Membrane Dyes Designed for Fixed CellsWhile classic lipophilic membrane dyes like DiI can be used to stain formaldehyde-fixed cells, staining can be highly variable due to the poor solubility of the dyes. CytoLiner™ Dyes are a new generation of membrane dyes uniquely engineered to permit selective staining of the plasma membrane in fixed and mildly permeabilized cells. CytoLiner™ staining is compatible with formaldehyde fixation and mild detergent permeabilization before staining. Staining can tolerate blocking agents used during immunofluorescence staining protocols, allowing subsequent staining with antibodies and other probes. The dyes also are compatible with poly-L-lysine coated cultureware and Transwell® permeable filter supports. CytoLiner™ Dyes are available in a selection of 5 colors from blue to near-IR.
Superior Staining Performance Over Classic Membrane Dyes
Comparison of fixed cell staining with CytoLiner™ Fixed Cell Membrane Stains versus classic lipophilic carbocyanine membrane dyes. In comparison to the traditional lipophilic carbocyanine dyes, CytoLiner™ dyes show more uniform and reliable staining of PFA-fixed, mildly-permeabilized cell membranes. PFA-fixed MCF7 cells were permeabilized for 10 minutes at RT with PBS/0.1% Triton® X-100, then rinsed with PBS and stained with carbocyanine dyes in PBS or CytoLiner™ Stains in 1X CytoLiner™ Buffer for 10 minutes at RT, then rinsed with PBS. Imaged by confocal microscopy; scale bar: 20 um.
Suitable for Co-Staining with Antibodies and Other Probes
PFA-fixed MCF-7 cells were permeabilized with PBS/0.1% Triton® X-100 for 10 minutes at room temperature, then stained with 1X CytoLiner™ Fixed Cell Membrane Dyes and NucSpot® Nuclear Stains.
CytoLiner™ Fixed Cell Membrane Stains
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| Source: | Synthetic |
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CytoLiner(TM) 650/675 |
