6 His epitope tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, His tag
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing f
Purity:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB:1:5000~10000 IP:1:50~100 IF:1:50~200
Application Notes:
His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6*His epitope tag.
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