Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.
Conjugation:
Unconjugated
Alternative Names:
Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3, SMAD3, MADH3, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolo
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function,
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
WB: 1:1000~1:2000 IF: 1:50~1:200 IP: 1:50~1:200
Application Notes:
p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.
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