Raf-1 (Phospho-Ser621) polyclonal antibody, Unconjugated, Rabbit

Product Overview

• Article Name: Raf-1 (Phospho-Ser621) polyclonal antibody, Unconjugated, Rabbit
• Biozol Catalog Number: BWT-AP0550
• Supplier Catalog Number: AP0550
• Alternative Catalog Number: BWT-AP0550-50UL,BWT-AP0550-100UL
• Manufacturer: Bioworld Technology
• Host: Rabbit
• Category: Antikörper
• Application: IF, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic phosphopeptide derived from human Raf-1 around the phosphorylation site of Serine 621.
• Conjugation: Unconjugated
• Alternative Names: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, RAF1, RAF

Product Description

A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites, including Ser338, Tyr341, Thr4

Product Properties

• Molecular Weight: ~ 75 kDa
• UniProt: P04049
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Form: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Application Notes

• Application Dilute: WB: 1:1000~1:2000 IF: 1:50~1:200 IP: 1:50~1:200
• Application Notes: Raf-1 (Phospho-Ser621) polyclonal antibody detects endogenous levels of Raf-1 protein only when phosphorylated at Ser621.