Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues.
Molecular Weight:
6.0 kDa+/-10% determined by reduced SDS-PAGE
Source:
Escherichia coli.
Purity:
Greater than 95% as determined by (a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Form:
Sterile Filtered White lyophilized (freeze-dried) powder.
Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.
Application Notes:
It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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