The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Application Dilute:
1:500 -1:5000 for immunohisto/cytochemistry 1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000-1:200,000 for Western blotting with ECL substrates
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