The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a
Purity:
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form:
Mouse IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Application Dilute:
IF/ICC: 1:50~1:200
Application Notes:
The specificity of the antibody has been tested by competitive ELISA. Binding was inhibited by 5-bromo-2-deoxyuridine (BrdU), 5-chloro-2-deoxyuridine (CldU) and 5-iodo-2-deoxyuridine (IdU). No cross-reactivity was observed with 5-fluoro-2-deoxyuridine (F
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