Recombinant protein rPhl p 5 is expressed in Escherichia coli. DNA sequence encoding 300 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 30 kDa.
Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 µm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
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