Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline p
filtered solution (30 kDa) in 10 mM Tris-HCl, Color: colorless to slightly yellow, pH: 7.5 0.5
Formula:
C31H54N7O16P3S
Application Notes:
Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data, Primer Extension with Klenow fragment. Spectroscopic Propertie: lambdamax 272 nm, epsilon 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
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