This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of the Nedd4 protein.
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Nedd4 protein. A BLAST analysis was used to suggest reactivity with Nedd4 from human, horse and macaque based on a 100%
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:20,000 - 1:35,000, IF: User Optimized, WB: 1:300 to 1:500
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Nedd4 protein. Specific conditions for reactivity and detection of Nedd4 should be optimized by the end user. Expect a band approximately ~115 kDa in
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