This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was
Form:
Liquid (sterile filtered)
Application Dilute:
ELISA: 1:5,000-1:16,000, WB: 1:2,500
Application Notes:
This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphoryl
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