Maximising RNA extraction from Buccal Swabs for Covid-19 Testing
Porvair Sciences and Finneran are supporting customers in critical research and development of Covid-19 testing by offering Spin Column Homogenizers for RNA extraction from Buccal swabs. Current diagnostic tests rely on the detection of viral RNA by reverse transcriptase polymerase chain reaction (RT-PCR) from Buccal swabs obtained from patient samples.
Sample preparation of nasal and throat Buccal swabs is essential to ensure high yields and quality of viral RNA for detection and analysis. The Chromatrap® Homogenizer Spin Column is a quick and rapid method to ensure samples are sufficiently prepared prior to RNA extraction to maximize downstream detection of viral RNA.
Whether you are researching Covid-19 to understand the mechanisms of action or front line diagnostic testing the Homogenizer Spin Column is the first step in high quality RNA extraction. The novel dual frit design reduces lysate viscosity and captures insoluble debris by centrifugation, the homogenized lysate sample is then ready for RNA extraction.
Collected Buccal swabs are placed in a Lysis/ Extraction Buffer and the lysate and swab transferred onto the Homogenizer Spin Column. After centrifugation at a high speed the resulting eluent contains homogenous RNA-extraction ready lysates. The Chromatrap® Homgenizer Spin Column provides a faster homogenization compared to syringe and needles, reduces waste and minimizes loss of sample. Every sample has its own homogenizer column and collection tube thus eliminating cross contamination between samples.
To get the best start to your experiments the Homogenizer Spin Column provides a fast procedure for downstream RNA extraction in minutes. The Chromatrap® Homogenizer Spin Column is compatible with all manual RNA extraction procedures including the Qiagen RNeasy and PureLink RNA kits.
The Chromatrap Homogeniser Spin columns quickly and efficiently homogenises cells and tissue lysates in a single step. They are a perfect alternative to syringe & needle or rotor-stator homogenization homogenisation and ideal sample preparation for nucleic acid miniprep and midiprep,or protein purification.
The spin columns contain two Porvair Sciences Vyon® discs designed and manufactured to exhibit low DNA/RNA and protein binding for bio-shredding applications, reducing lysate viscosity by removing and capturing high molecular weight insoluble components. The Chromatrap Homogeniser Spin columns are therefore easy to use, reliable and offer high sample recovery, with no sample to sample cross contamination or contamination from the spin column.
Addressable applications
Cell/tissue sample homogenisation
RNA extraction
DNA extraction
Protein lysate prep
Enzyme assay
Protocol
1. Load the sample lysate into Chromatrap Homogeniser Spin column column with a collection tube.
2. Spin at full speed in a micro-centrifuge. (See spec table below)
3. Filtered lysate is collected in the 2ml collection tube, Homogeniser top section is discarded.
4. Samples can then be removed into a fresh storage tube for required application.
* VAT and and shipping costs not included. Errors and price changes excepted
