Ras activation by EGF measured by G-LISA™. HeLa cells were serum starved (SS) for 24 h and treated with EGF (100 ng/ml for 2 min). 25, 12.5, 5, 1.25 µg of cell lysates were subjected to the G-LISA™ assay. Absorbance was read at 490 nm. Data are background subtracted
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