Anti-CD31 (Ms,Sw) from Rat (SZ31) for mouse FFPE tissue

Product Overview

• Article Name: Anti-CD31 (Ms,Sw) from Rat (SZ31) for mouse FFPE tissue
• Biozol Catalog Number: DNA-DIA-310
• Supplier Catalog Number: DNA-DIA-310
• Alternative Catalog Number: DNA-DIA-310
• Manufacturer: dianova
• Host: Rat
• Category: Antikörper
• Application: IHC
• Species Reactivity: Mouse, Sw
• Immunogen: Murines Aminosaure-Fragment (Aminosauren 610-681 von Maus CD31)
• Conjugation: Unconjugated
• Alternative Names: Platelet endothelial cell adhesion molecule,PECAM-1,EndoCAM,GPIIA,PECA1,CD31,PECAM1,Platelet/Endothelial Cell Adhesion Molecule 1,Platelet/Endothelial Cell Adhesion Molecule,PECAM1,Pecam,PECAM-1

Product Description

Anti-CD31 (Ms,Sw) aus Ratte (SZ31) für Paraffingewebe aus Maus, IgG2a, Unconjugated, Monoclonal

Additional Information

Antibody clone SZ31 is the first antibody which reacts specifically with murine CD31 in formalin-fixed paraffin-embedded tissue sections.

 

Background

CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1) is expressed constitutively on the surface of embryonic and adult endothelial cells. It is also expressed on cell surfaces of monocytes, neutrophils, platelets and certain T-cell subsets. It has been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is a 130kDa integral membrane glycoprotein and as a member of the immunoglobulin superfamily involved in the mediation of cell-to-cell adhesion. CD31-mediated endothelial cell-cell interactions play a major role in angiogenesis. Studies have shown CD31 to be a superior marker in human angiogenesis, which reportedly predicts tumor recurrence. Pathophysiological studies of CD31 in murine model systems had limitations because standard formalin-fixed sections were excluded. The clone SZ31 eliminates these restrictions by allowing high quality immunohistochemical (IHC) analysis of standard formalin-fixed paraffin sections in mice.

 

Instructions for Use

Immunohistochemical staining of standard formalin-fixed paraffin sections Indirect alkaline phosphatase staining (Other techniques, e.g. Avidin-Biotin-alkaline phosphatase (ABAP), alkaline phosphatase anti-alkaline phosphatase (APAAP) or horseradish peroxidase (HRP) -method are also possible).

1. Deparaffinize formalin-fixed paraffin-embedded mouse tissue sections by a standard procedure using xylol/ethanol

2. Antigen retrieval: high temperature heating of sections in citrate buffer pH 6,0 according to standard procedures

3. Block with 5% rabbit serum, 10 min RT

4. Wash with TBS, 3 x 5 min

5. Incubate with DIA-310 (1:10-1:20), 30min RT

6. Wash with TBS, 3 x 5 min

7. Incubate with rabbit anti-rat IgG (H+L) alkaline phosphatase (1:200), 30min RT

8. Wash with TBS, 3 x 5 min

9. Add substrate, e.g. Neufuchsin, 30min RT

10. Counterstain, e.g. with Hematoxylin-Papanicolaou

 

Product Specification

• Artikelnummer: DNA-DIA-310
• Spezifität:

  CD31 (PECAM-1)

• Spezies-Reaktivität:

  Maus, Schwein

• Immunogen:

  Murines Aminosäure-Fragment (Aminosäuren 610-681 von Maus CD31)

• Wirtsspezies:

  Ratte

• Isotyp:

  IgG2a

• Klon:

  SZ31

• Klonalität (Mono-/Polyklonal):

  monoklonal

• Anwendung:

  Immuncytochemie, Immunfluoreszenz, Immunhistochemie (Gefrierschnitte), Immunhistochemie (IHC), Immunhistochemie (Paraffingewebe), Western Blot

• Konjugation:

  unkonjugiert

• Verdünnung:

  Immunhistochemie (IHC): 1:20, Western Blot (WB): 1:5.000

• Format:

  0,05% NaN3, 2% BSA, gereinigter Antikörper (aus Kulturüberstand), in PBS (pH 7,4), Lyophilisat

• Anwendungshinweis:

  Keine Kreuzreaktivität human CD31

• Produktlinie / Thema:

  Angiogenese, CD-Marker

• Zweckbestimmung:

  nur für Forschungszwecke

• Temperatur - Lagerung:

  2-8°C

• Temperatur - Transport:

  bei Raumtemperatur

• Suchcode:

  DIA310

• Hersteller / Marke:

  dianova

• Uniprot_ID:

  Q08481

• Gene_ID:

  18613

• Alias:

  CD31, EndoCAM, GPIIA', PECA1, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/Endothelial Cell Adhesion Molecule, Platelet/Endothelial Cell Adhesion Molecule 1

Product Properties

• Clonality: Monoclonal
• Concentration: (0,2mg/ml)
• Clone Designation: SZ31
• Isotype: IgG2a
• NCBI: 18613
• UniProt: Q08481
• Buffer: in PBS, 2% BSA, 0,05%NaN3
• Storage: 2-8 °C
• Target: CD31

Application Notes

• Application Dilute: Immunhistochemie (IHC): 1:20,Western Blot (WB): 1:5.000

Images

Mouse Aorta (FFPE): anti-CD31 staining with clone SZ31

Mouse Endocardium (FFPE): anti-CD31 staining with clone SZ31

Murine adenocarcinoma. anti-CD31 staining with clone SZ31

Murine Spinal Cord: anti-CD31 staining with clone SZ31

Western Blot of murine cell lysate with antibody clone SZ31

Aortic-origin CD31 staining with clone SZ31

Bone-Marrow (IHC-P) - Staining with anti-CD31 Antibody clone SZ31

Mouse Brain (IHC-P) - Staining with anti-CD31 Antibody

murine Colon (IHC-P) - Staining with anti-CD31 Antibody (SZ31)

murine Kidney (IHC-P) - Staining with anti-CD31 Antibody (SZ31)

murine Liver (IHC-P) - Staining with anti-CD31 Antibody (SZ31)

Mouse Lung CD31 Staining Antibody SZ31

Mouse Lymph-nodes

Mosue Mesenteric vessels

Murine adenocarcinoma. anti-CD31 staining with clone SZ31 (higher magnification)

Mouse Skeletal muscle

Mouse Pancreas (FFPE)

CD31 in Small intestine (Ms) - Staining with anti-CD31)

CD31 in Small intestine (Ms) (higher magnification) (Staining with anti-CD31)