The rapid photobleaching of cell probes labelled with fluorochromes has always hindered the qualitative and quantitative assessment of the label. ImmunoSelect® Antifading Mounting Medium is developed for fluorescence-microscopical use combining strong initial fluorescence with drastical reduction of photobleaching over time. In contrast to several media ImmunoSelect® Antifading Mounting Medium does not exhibit autofluorescence at any common wavelength. ImmunoSelect® Antifading Mounting Medium is an aqueous mountant which does not solidify directly, but remains a viscous liquid for several days on the slide. To mount cellular material on the slide one drop of medium is sufficient. After a cover slip is applied, the medium disperses over the entire section. When mounted slides are stored at 4°C in the dark, fluorescence will remain for many weeks.
ImmunoSelect® Antifading Mounting Medium is ready to use and available with or without DNA Fluorescent Dyes (DAPI or PI (propidium iodide)).
Immunoselect Antifading Mounting Medium is tested for several fluorescence dyes:
• Fluorescein derivatives, eg. FITC
• Rhodamine derivatives, eg. Rhodamin Red-X, TRITC, Texas Red
• All common fluorescent dyes, eg. Alexa Fluor dyes, DyLight dyes, Cyanine dyes
• Phycobilliproteins, eg. PE
• DAPI
• Hoechst 33358 and 3334
• Propidium Iodide
Storage:
2-8°C
Caution:
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Colocalization of SP-C (red) and GFAP (green) in the endothelium of cortical vessels in rat brain; Mounting with Immunoselect Antifading Mounting Medium; 40x Objektiv. (Image curtesy of N. Peukert, Polyclinic for Paediatric Surgery, University of Leipzig, Germany)
Immunostaining of DNA adducts (red) and CNPase (green) in cryosections of the inner ear (stria vascularis) from mice (24 h after exposure to a DNA-reative platinum compound). (Image curtesy of J. Thomale, Institute for Cell Biology, University of Duisburg-Essen, Germany)
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