The rapid photobleaching of fluorochrome-labeled cell probes has always been an obstacle to the qualitative and quantitative assessment of immnunofluorescent assays. ImmunoSelect® Antifading Mounting Medium w/o Hardener is designed for fluorescence microscopy applications and combines strong initial fluorescence with dramatic reduction of photobleaching over time. Unlike some media, ImmunoSelect® Antifading Mounting Medium does not exhibit autofluorescence at any common wavelength.
ImmunoSelect® Antifading Mounting Medium w/o Hardener is an aqueous mounting medium that contains no hardener and remains a viscous liquid. One drop of the medium is sufficient to mount cellular material onto the slide. When a coverslip is applied, the medium will spread over the entire section. Seal the coverslip to the slide with clear nail polish. If mounted slides are stored at 4°C in the dark, fluorescence will persist for many weeks.
ImmunoSelect® Antifading Mounting Medium is ready to use and available with or without DNA Fluorescent Dyes (DAPI or PI (propidium iodide)).
Immunoselect Antifading Mounting Medium is tested for several fluorescence dyes:
• Fluorescein derivatives, eg. FITC
• Rhodamine derivatives, eg. Rhodamin Red-X, TRITC, Texas Red
• All common fluorescent dyes, eg. Alexa Fluor dyes, DyLight dyes, Cyanine dyes
• Phycobilliproteins, eg. PE
• DAPI
• Hoechst 33358 and 3334
• Propidium Iodide
Storage:
2-8°C
Caution:
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Colocalization of SP-C (red) and GFAP (green) in the endothelium of cortical vessels in rat brain; Mounting with Immunoselect Antifading Mounting Medium; 40x Objektiv. (Image curtesy of N. Peukert, Polyclinic for Paediatric Surgery, University of Leipzig, Germany)
Immunostaining of DNA adducts (red) and CNPase (green) in cryosections of the inner ear (stria vascularis) from mice (24 h after exposure to a DNA-reative platinum compound). (Image curtesy of J. Thomale, Institute for Cell Biology, University of Duisburg-Essen, Germany)
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