Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
Conjugation:
Unconjugated
Alternative Names:
Human Antibody, Rb-a-Human IgG, Human Antibody in Rabbit, Human Secondary Antibody
Anti-Human IgG (H&L) generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light
Clonality:
Polyclonal
Concentration:
10.0 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by
Formula:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,Azide/BSA free
Target:
Human
Antibody Type:
Polyclonal Antibody
Application Dilute:
WB: 1:2,000 - 1:10,000
Application Notes:
Anti-Human IgG antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.
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