Anti-Human IgG was produced by repeated immunization with human IgG whole molecule fragment in rabbit.
Conjugation:
Unconjugated
Alternative Names:
Human IgG (H&L) Antibody, Rabbit-a-Human IgG (H&L), Human IgG (H&L) Antibody in rabbit, Rabbit anti-human IgG Secondary Antibody.
Anti-Human IgG (H&L) generated in rabbit detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light
Clonality:
Polyclonal
Concentration:
2.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Human
Antibody Type:
Polyclonal Antibody
Application Dilute:
WB: 1:10,000 - 1:50,000
Application Notes:
Anti-Human IgG (H&L) has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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