Anti-Rat IgG (H&L) generated in chicken detects rat Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the ci
Clonality:
Polyclonal
Concentration:
1.2 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single p
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,01% NaN3
Target:
Rat
Antibody Type:
Polyclonal Antibody
Application Dilute:
WB: 1:500 - 1:2,500
Application Notes:
Anti-Rat IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) code NPP-10 as a substrate for 30 minutes at room temperature. A wo
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