Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.
Conjugation:
Unconjugated
Alternative Names:
Rabbit Anti-Sheep IgG F(c) Antibody, Rabbit Anti-Sheep IgG Fc Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody
Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of sheep IgG and often
Clonality:
Polyclonal
Concentration:
2.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Sheep
Antibody Type:
Polyclonal Antibody
Application Dilute:
WB: 1:1,000 - 1:5,000
Application Notes:
Anti-Sheep IgG F(c) antibody has been tested by ELISA and dot blot and is assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at
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