iLite Insulin*
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Article Name: | iLite Insulin* |
Biozol Catalog Number: | EUD-BM3060 |
Supplier Catalog Number: | BM3060 |
Alternative Catalog Number: | EUD-BM3060 |
Manufacturer: | Svar Life Science |
Category: | Humandiagnostik |
The insulin-responsive iLite® Insulin Assay Ready Cells provide the basis for the establishment of a rapid, sensitive, and highly specific assay for the quantification of insulin activity that can be completed within 6 hours. iLite Insulin assay ready cells are avian DT-40 cells (ATCC#CRL211) which has been genetically engineered to be responsive to Insulin, resulting in the specific expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor. The assay can be used to quantify the activity of insulin or an insulin analog in human serum without interference from factors present in human serum, including IGF-1. In addition, iLite assay ready cells can be used for measuring the presence of neutralizing anti-insulin antibodies. The ATCC® CRL-2111 cell line is used under a license from ATCC® FOR RESEARCH USE ONLY |
Form: | Assay Ready Cells |