Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and as
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[Hs-14]
Isotype:
Mouse IgM
Buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
VCP
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunocytochemistry: Recommended dilution: 10 µg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reprodu
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