Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP c
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[Hs-36]
Isotype:
IgM
Buffer:
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage:
2°C to 8°C
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunocytochemistry: Recommended dilution: 10 µg/ml, membrane permeabilization (acetone) is essential.
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