Mouse IgG2b Isotype Control Alexa Fluor 488, Clone: [MPC-11], AF488, Monoclonal

Product Overview

• Article Name: Mouse IgG2b Isotype Control Alexa Fluor 488, Clone: [MPC-11], AF488, Monoclonal
• Biozol Catalog Number: EXB-A4-692-C100
• Supplier Catalog Number: A4-692-C100
• Alternative Catalog Number: EXB-A4-692-C100
• Manufacturer: EXBIO
• Category: Antikörper
• Application: FC, ICC, IHC-Fr, IHC-P, WB
• Species Reactivity: Mouse, Rat, Human
• Immunogen: KLH-coupled trinitrophenol
• Conjugation: AF488

Additional Information

Applikation details:

Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Product Specific References:

*Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type-1 of CD8-positive T cells. World J Gastroenterol. 2010 Feb 7;16(5):618-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/20128032 ,*Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues. Vet Res. 2011 Aug 22;42(1):95., URL: https://pubmed.ncbi.nlm.nih.gov/21859474/,*Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14-dependent activation of transcription factors CREB/ATF and AP-1. J Biol Chem. 1999 May 14;274(20):14012-20., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=gupta%20d%20vinson%20c ,*Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008 May 15;111(10):5037-46., URL: http://www.ncbi.nlm.nih.gov/pubmed/18337560 ,*Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J Immunol. 2006 Oct 15;177(8):5226-35., URL: https://pubmed.ncbi.nlm.nih.gov/17015708/,

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml
• Clone Designation: [MPC-11]
• Isotype: Mouse IgG2b kappa
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Storage: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Target: This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the