BasoFlowEx Kit

Product Overview

• Article Name: BasoFlowEx Kit
• Biozol Catalog Number: EXB-ED7043
• Supplier Catalog Number: ED7043
• Alternative Catalog Number: EXB-ED7043
• Manufacturer: EXBIO
• Category: Kits/Assays
• Application: FC

Product Description

The BasoFlowEx Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation. Commercially available allergens can be

Additional Information

 

The BasoFlowEx Kit (CE IVD) is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation.
Commercially available allergens can be used for the stimulation.

The product is intended for In Vitro Diagnostic Use. 

 

Application

Flow Cytometry

Reactivity

Human

Content

ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes.
ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls.
ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled.
ED7043-4 Lysing Solution – 30 ml.

Product Properties

• Storage: 2°C to 8°C

Application Notes

• Application Notes: FC (QC tested)

Images

Content ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes. ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls. ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled. ED7043-4 Lysing Solution – 30 ml.

Flow cytometric analysis of BasoFlowEx Kit. (A) Visualization of compensated data on the side-scatter (SSC) versus forward scatter (FSC) in dot-plot. (B) Visualization of single cells gate on the FSC-Area versus FSC-height in dot-plot. (C) Visualization of basophil population (CD203 positive versus CD63 positive in dot-plot). (D) Visualization of stimulated (shown in red) and non-stimulated basophil population (shown in dashed line).