4 µM HOP Protein, when added to 2 µM AHA1 Protein (Aha1)-activated HSP90 (2 µM, His-tagged HSP90 beta) in 33 mM HEPES (pH 7.2) with 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37 C, eliminated all Aha1-mediated ATPase stimulatio
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