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GTX00697 ICC/IF Image |
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ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10]. Dilution : 10 μg/ml Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min Permeabilization : 1% Triton X-100 for 1 min |
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ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10]. Dilution : 10 μg/ml Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min Permeabilization : 1% Triton X-100 for 1 min |
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ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10]. The dots are Nup98. |
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WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10]. Dilution : 1 μg/ml |
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WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10]. Dilution : 1:2000 |
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ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10]. Dilution : 0.5 μg/ml Fixation : Cold Methanol (-30 degree C) for 30 min |
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Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications. |
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WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10]. Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe. Dilution : 2 μg/ml |