NUP98 antibody [2H10], IgG2c, Unconjugated, Rat, Monoclonal

Catalog Number: GTX00697
Article Name: NUP98 antibody [2H10], IgG2c, Unconjugated, Rat, Monoclonal
Biozol Catalog Number: GTX00697
Supplier Catalog Number: GTX00697
Alternative Catalog Number: GTX00697-100
Manufacturer: GeneTex
Host: Rat
Category: Antikörper
Application: DOT, ELISA, ICC, WB
Species Reactivity: Fungi, Human, Mouse, Rat, Yeast
Immunogen: Recombinant GST-fused human Nup98 (amino acids 1-466)
Conjugation: Unconjugated
Alternative Names: nucleoporin 98 , ADIR2 , NUP196 , NUP96
Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and
Clonality: Monoclonal
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: [2H10]
Molecular Weight: 198
Isotype: IgG2c
Sensitivity: We do not recommend use of this product for Tetrahymena.
NCBI: 4928
Pubmed: 23607342, 16225424,
UniProt: P52948
Buffer: Filter-sterilized PBS, 50% Glycerol, no preservative.
Source: Human
Purity: Purified IgG
Form: Liquid
Application Notes: *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX00697 ICC/IF Image
ICC/IF analysis of S. cereviciae cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of S. pombe cells using GTX00697 NUP98 antibody [2H10].
Dilution : 10 μg/ml
Fixation : 4% PFA for 10 min followed by 0.6 mg/ml Zymolyase 100T treatment at 36 degree C for 70 min
Permeabilization : 1% Triton X-100 for 1 min
ICC/IF analysis of rat neuron cells using GTX00697 NUP98 antibody [2H10].
The dots are Nup98.
WB analysis of S. pombe cell extracts expressing wilde type Nup98 (Lane 1) or Nup98-GFP protein (Lane 2) using GTX00697 NUP98 antibody [2H10].
Dilution : 1 μg/ml
WB analysis of HeLa nuclear membrane extract using GTX00697 NUP98 antibody [2H10].
Dilution : 1:2000
ICC/IF analysis of HeLa cells using GTX00697 NUP98 antibody [2H10].
Dilution : 0.5 μg/ml
Fixation : Cold Methanol (-30 degree C) for 30 min
Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
WB analysis of S. cereviciae cell extracts using GTX00697 NUP98 antibody [2H10].
Five protein bands identified by this antibody correspond to the sizes of 5 kinds of nucleoporins with multiple GFLG motifs in S. pombe.
Dilution : 2 μg/ml