Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22µm-filtered PBS (pH7.4), no preservative.
Expression System:
HEK293 cells
Form:
Lyophilized powder
Sequence:
Val16-Arg685 of SARS-CoV-2 (COVID-19) Spike S1 protein (Accession YP_009724390.1) fused with a 6*His tag at the C-terminus.
Application Notes:
Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 µg/ml (100 µl/well ) can bind Recombinant nCoV-S1. The EC50 of SARS-CoV-2 (COVID-19) Spike S1 protein is 0.25-0.45 µg/ml.
SDS-PAGE of GTX01554-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active).
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
GTX01554-pro ELISA Image
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
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