Syk antibody [N2C2], Internal, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Syk antibody [N2C2], Internal, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX100748
• Supplier Catalog Number: GTX100748
• Alternative Catalog Number: GTX100748-100,GTX100748-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: spleen associated tyrosine kinase , p72-Syk

Product Properties

• Clonality: Polyclonal
• Concentration: 0.32 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 72
• NCBI: 6850
• UniProt: P43405
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX100748 WB Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using SYK(GTX100748) antibody at 1:200 dilution.

SYK antibody [N2C2], Internal detects SYK protein at cytosol on rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 μg of SYK antibody [N2C2] (GTX100748).
Western blot analysis was performed using SYK antibody [N2C2] (GTX100748).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SYK antibody detects SYK protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.