alpha Cardiac Muscle Actin antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX101876
Article Name: alpha Cardiac Muscle Actin antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX101876
Supplier Catalog Number: GTX101876
Alternative Catalog Number: GTX101876-100,GTX101876-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ICC, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Cardiac Muscle Actin. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: actin alpha cardiac muscle 1 , ACTC , ASD5 , CMD1R , CMH11 , LVNC4
Clonality: Polyclonal
Concentration: 1.71 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight: 42
NCBI: 70
UniProt: P68032
Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX101876 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Cardiac Muscle Actin antibody (GTX101876) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
alpha Cardiac Muscle Actin stained by alpha Cardiac Muscle Actin antibody (GTX101876) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
alpha Cardiac Muscle Actin stained by alpha Cardiac Muscle Actin antibody (GTX101876) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Cardiac Muscle Actin antibody (GTX101876) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein by western blot analysis. Various whole cell extracts (30 μg) and Rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Cardiac Muscle Actin antibody (GTX101876) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (GTX101876) diluted at 1:200.
Blue: Hoechst 33342 staining.
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
alpha Actin (cardiac muscle) antibody (GTX101876) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.