GRP94 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GRP94 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX103232
• Supplier Catalog Number: GTX103232
• Alternative Catalog Number: GTX103232-100,GTX103232-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GRP94. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 92
• NCBI: 7184
• UniProt: P14625
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX103232 IHC-P Image

GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000

Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94(GTX103232) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GRP94 protein stained by GRP94 antibody (GTX103232) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody (GTX103232).
Western blot analysis was performed using GRP94 antibody (GTX103232).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94(GTX103232) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min