Calretinin antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Calretinin antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX103261
• Supplier Catalog Number: GTX103261
• Alternative Catalog Number: GTX103261-100,GTX103261-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: calbindin 2 , CAB29 , CAL2 , CR

Product Properties

• Clonality: Polyclonal
• Concentration: 1.08 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 32
• NCBI: 794
• UniProt: P22676
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX103261 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody (GTX103261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX103261 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calretinin antibody detects Calretinin protein at cytoplasm by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:200.
Blue: Hoechst 33342 staining.

Calretinin antibody detects Calretinin protein by immunohistochemical analysis.
Samples: Frozen Section adult mouse hippocampus.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Calretinin antibody detects CALB2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human CALB2-transfected 293T cells
12% SDS-PAGE
Calretinin antibody (GTX103261) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin(GTX103261) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Calretinin antibody detects Calretinin protein in the amacrine cells by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Calretinin protein stained by Calretinin antibody (GTX103261) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
Calretinin antibody (GTX103261) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min