Wnt11 antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX105971
Article Name: Wnt11 antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX105971
Supplier Catalog Number: GTX105971
Alternative Catalog Number: GTX105971-100,GTX105971-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ICC, IHC, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human WNT11. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: Wnt family member 11 , HWNT11
Clonality: Polyclonal
Concentration: 1.39 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight: 39
NCBI: 7481
UniProt: O96014
Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX105971 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using WNT11(GTX105971) antibody at 1:200 dilution.
WNT11 antibody detects WNT11 protein at secreted on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
WNT11 antibody (GTX105971) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using WNT11(GTX105971) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt11 antibody (GTX105971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.