WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX108777 WB Image
CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse placenta.Green: CD146 stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CD146 antibody [C1C3] (GTX108777) dilution: 1:500.
CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500. Blue: Hoechst 33342 staining.
CD146 antibody [C1C3] detects CD146 protein by western blot analysis. A. 50 μg rat liver extract 7.5 % SDS-PAGE CD146 antibody [C1C3] (GTX108777) dilution: 1:1000
CD146 antibody [C1C3] detects CD146 protein by Western blot analysis. A. 30 μg HUVEC whole cell lysate/extract 7.5 % SDS-PAGE CD146 antibody [C1C3] (GTX108777) dilution: 1:10000
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CD146 antibody [C1C3] (GTX108777) dilution: 1:500. _x000D_ Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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