CD146 antibody [C1C3], Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX108777
Article Name: CD146 antibody [C1C3], Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX108777
Supplier Catalog Number: GTX108777
Alternative Catalog Number: GTX108777-100,GTX108777-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ICC, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human CD146. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: melanoma cell adhesion molecule , CD146 , HEMCAM , METCAM , MUC18 , MelCAM
Clonality: Polyclonal
Concentration: 0.21 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight: 72
NCBI: 4162
UniProt: P43121
Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX108777 WB Image
CD146 antibody [C1C3] detects CD146 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
Green: CD146 stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:250.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded J5 xenograft, using CD146(GTX108777) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
CD146 antibody [C1C3] detects CD146 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HUVEC cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD146 protein stained by CD146 antibody [C1C3] (GTX108777) diluted at 1:500.
Blue: Hoechst 33342 staining.
CD146 antibody [C1C3] detects CD146 protein by western blot analysis.
A. 50 μg rat liver extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:1000
CD146 antibody [C1C3] detects CD146 protein by Western blot analysis.
A. 30 μg HUVEC whole cell lysate/extract
7.5 % SDS-PAGE
CD146 antibody [C1C3] (GTX108777) dilution: 1:10000
CD146 antibody [C1C3] detects CD146 protein at membrane on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CD146 antibody [C1C3] (GTX108777) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD146 antibody [C1C3] (GTX108777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.