WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX109753 IHC-P Image
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Hsp90 alpha antibody (GTX109753) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp90 alpha antibody (GTX109753) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Hsp90 alpha protein from 293T whole cell extracts using 5 μg of Hsp90 alpha antibody (GTX109753). Western blot analysis was performed using Hsp90 alpha antibody (GTX109753). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Hsp90 alpha antibody detects Hsp90 alpha protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hsp90 alpha stained by Hsp90 alpha antibody (GTX109753) diluted at 1:500.Blue: Hoechst 33342 staining.
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