WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX110593 IHC-P Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
ChIP was performed with 293T chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 μg of OCT1 antibody (GTX110593). Western blot analysis was performed using OCT1 antibody (GTX110593) diluted at 1:250. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SP1(GTX110593) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SP1 stained by SP1 antibody (GTX110593) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SP1 stained by SP1 antibody (GTX110593) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
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