WB: 1:500-1:20000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX111010 WB Image
Sample (30 μg of whole cell lysate) A: Raji 15% SDS PAGE GTX111010 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FIS1 antibody detects FIS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: mouse brain 15% SDS PAGE GTX111010 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FIS1 antibody detects FIS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: FIS1 protein stained by FIS1 antibody (GTX111010) diluted at 1:2000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
TTC11 antibody detects TTC11 protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. TTC11 antibody (GTX111010) dilution: 1:500.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:10000.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using FIS1(GTX111010) antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FIS1 antibody (GTX111010) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
* VAT and and shipping costs not included. Errors and price changes excepted