WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX111294 IP Image
RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. RUVBL1 antibody (GTX111294) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.
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