WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX112815 ICC/IF Image
Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments. IP samples: HeLa whole cell extract A. 30 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815) 15 % SDS-PAGE The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.
beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE GTX112815 diluted at 1:5000
Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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